Shi, S. H., Hayashi, Y., Petralia, R. S., Zaman, S. H., Wenthold, R. J., Svoboda, K., Malinow, R.
(June 1999)
Rapid spine delivery and redistribution of AMPA receptors after synaptic NMDA receptor activation.
Science, 284 (5421).
pp. 1811-6.
ISSN 0036-8075 (Print)
Abstract
To monitor changes in alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptor distribution in living neurons, the AMPA receptor subunit GluR1 was tagged with green fluorescent protein (GFP). This protein (GluR1-GFP) was functional and was transiently expressed in hippocampal CA1 neurons. In dendrites visualized with two-photon laser scanning microscopy or electron microscopy, most of the GluR1-GFP was intracellular, mimicking endogenous GluR1 distribution. Tetanic synaptic stimulation induced a rapid delivery of tagged receptors into dendritic spines as well as clusters in dendrites. These postsynaptic trafficking events required synaptic N-methyl-D-aspartate (NMDA) receptor activation and may contribute to the enhanced AMPA receptor-mediatedtransmission observed during long-term potentiation and activity-dependent synaptic maturation.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Animals
Cells, Cultured
Dendrites/ metabolism/ultrastructure
Electric Stimulation
Hippocampus/cytology/physiology
Humans
Long-Term Potentiation
Neuronal Plasticity
Neurons/ physiology
Organ Culture Techniques
Rats
Receptor Aggregation
Receptors, AMPA/ metabolism
Receptors, N-Methyl-D-Aspartate/ physiology
Recombinant Fusion Proteins/metabolism
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Synapses/metabolism/ physiology
Synaptic Transmission
Tetany |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > Long term potentiation
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > synaptic transmission |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Malinow lab
CSHL labs > Svoboda lab |
| Depositing User: |
Kathleen Darby
|
| Date: |
11 June 1999 |
| Date Deposited: |
23 Apr 2014 20:27 |
| Last Modified: |
23 Apr 2014 20:27 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/29824 |
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