MaRX: an approach to genetics in mammalian cells

Hannon, G. J., Sun, P., Carnero, A., Xie, L. Y., Maestro, R., Conklin, D. S., Beach, D. (February 1999) MaRX: an approach to genetics in mammalian cells. Science, 283 (5405). pp. 1129-30. ISSN 0036-8075

URL: http://www.ncbi.nlm.nih.gov/pubmed/10075573
DOI: 10.1126/science.283.5405.1129

Abstract

A genetic approach is the most direct way to elucidate biological processes that are poorly understood. One of the first such efforts—the landmark study of Beadle and Tatum (1) on the genetics of metabolic pathways—established the influential “one gene, one enzyme” hypothesis. In subsequent decades, the yeasts Saccharomyces cerevisiae and S. pombe became the premier genetic models. The oft-touted “power of yeast genetics” was not fully realized, however, until classical techniques were combined with an ability to manipulate the organisms with recombinant DNA methods (2–4). Thus were conceived the tools that today make yeasts the best-characterized eukaryotes. These tools, however, have limitations: accumulating human sequence data reveals many genes that are not represented in yeast. How can the leap be made from yeast to human?

Item Type: Paper
Additional Information: 0036-8075 (Print) Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.
Uncontrolled Keywords: Animals Cell Line Cloning, Molecular/*methods DNA, Complementary Gene Expression Gene Library Genes, p53 Genes, ras *Genetic Techniques Genetic Vectors Mammals Phenotype Proviruses/genetics Retroviridae/genetics Transformation, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line

Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > p53
CSHL Authors:
Communities: CSHL labs
CSHL labs > Hannon lab
CSHL labs > Beach lab
Depositing User: Kathleen Darby
Date: 19 February 1999
Date Deposited: 30 Apr 2014 13:23
Last Modified: 05 May 2014 16:55
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29799

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