Driving AMPA receptors into synapses by LTP and CaMKII: Requirement for GluR1 and PDZ domain interaction

Hayashi, Y., Shi, S. H., Esteban, J. A., Piccini, A., Poncer, J. C., Malinow, R. (March 2000) Driving AMPA receptors into synapses by LTP and CaMKII: Requirement for GluR1 and PDZ domain interaction. Science, 287 (5461). pp. 2262-2267. ISSN 0036-8075

URL: http://www.ncbi.nlm.nih.gov/pubmed/10731148
DOI: 10.1126/science.287.5461.2262

Abstract

To elucidate mechanisms that control and execute activity-dependent synaptic plasticity, alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptors (AMPA-Rs) with an electrophysiological tag were expressed in rat hippocampal neurons. Long-term potentiation (LTP) or increased activity of the calcium/calmodulin-dependent protein kinase II (CaMKII) induced delivery of tagged AMPA-Rs into synapses. This effect was not diminished by mutating the CaMKII phosphorylation site on the GLuR1 AMPA-R subunit, but was blocked by mutating a predicted PDZ domain interaction site. These results show that LTP and CaMKII activity drive AMPA-Rs to synapses by a mechanism that requires the association between GluR1 and a PDZ domain protein.

Item Type: Paper
Uncontrolled Keywords: LONG-TERM POTENTIATION DEPENDENT PROTEIN-KINASE SYNAPTIC TRANSMISSION GLUTAMATE RECEPTORS IN-VIVO CALMODULIN NEURONS PHOSPHORYLATION MECHANISMS COMPONENT
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > CaMKII
bioinformatics > genomics and proteomics > small molecules > Glutamate
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
organism description > animal behavior > memory
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > synaptic plasticity
CSHL Authors:
Communities: CSHL labs > Malinow lab
Depositing User: Matt Covey
Date: March 2000
Date Deposited: 31 Jan 2014 16:08
Last Modified: 31 Jan 2014 16:08
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29380

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