Inhibition of Ras-induced DNA synthesis by expression of the phosphatase MKP-1

Sun, H., Tonks, N. K., Barsagi, D. (October 1994) Inhibition of Ras-induced DNA synthesis by expression of the phosphatase MKP-1. Science, 266 (5183). pp. 285-288. ISSN 0036-8075

URL: http://www.ncbi.nlm.nih.gov/pubmed/7939666
DOI: 10.1126/science.7939666

Abstract

Mitogen-activated protein kinases (MAP kinases) are common components of signaling pathways induced by diverse growth stimuli. Although the guanidine nucleotide-binding Ras proteins are known to be upstream activators of MAP kinases, the extent to which MAP kinases directly contribute to the mitogenic effect of Ras is as yet undefined. In this study, inhibition of MAP kinases by the MAP kinase phosphatase MKP-1 blocked the induction of DNA synthesis in quiescent rat embryonic fibroblast REF-52 cells by an activated mutant of Ras, V(12)Ras. These results suggest an essential role for activation of MAP kinases in the transition from the quiescent to the DNA replication phase of the eukaryotic cell cycle.

Item Type: Paper
Uncontrolled Keywords: ACTIVATED PROTEIN-KINASES IMMEDIATE-EARLY GENE MAP KINASE SIGNAL-TRANSDUCTION PHOSPHORYLATION PROLIFERATION PATHWAY DOMAIN
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > G protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > immediate early genes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > protein phosphatase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > G protein > Ras
CSHL Authors:
Communities: CSHL labs > Tonks lab
Depositing User: Matt Covey
Date: October 1994
Date Deposited: 18 Dec 2013 15:19
Last Modified: 18 Dec 2013 15:19
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29064

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