Lsd1 and Lsd2 Control Programmed Replication Fork Pauses and Imprinting in Fission Yeast

Holmes, A., Roseaulin, L., Schurra, C., Waxin, H., Lambert, S., Zaratiegui, M., Martienssen, R. A., Arcangioli, B. (December 2012) Lsd1 and Lsd2 Control Programmed Replication Fork Pauses and Imprinting in Fission Yeast. Cell Reports, 2 (6). pp. 1513-1520. ISSN 2211-1247

URL: http://www.ncbi.nlm.nih.gov/pubmed/23260662
DOI: 10.1016/j.celrep.2012.10.011

Abstract

In the fission yeast Schizosaccharomyces pombe, a chromosomal imprinting event controls the asymmetric pattern of mating-type switching. The orientation of DNA replication at the mating-type locus is instrumental in this process. However, the factors leading to imprinting are not fully identified and the mechanism is poorly understood. Here, we show that the replication fork pause at the mat1 locus (MPS1), essential for imprint formation, depends on the lysine-specific demethylase Lsd1. We demonstrate that either Lsd1 or Lsd2 amine oxidase activity is required for these processes, working upstream of the imprinting factors Swi1 and Swi3 (homologs of mammalian Timeless and Tipin, respectively). We also show that the Lsd1/2 complex controls the replication fork terminators, within the rDNA repeats. These findings reveal a role for the Lsd1/2 demethylases in controlling polar replication fork progression, imprint formation, and subsequent asymmetric cell divisions.

Item Type: Paper
Uncontrolled Keywords: ribosomal-rna genes mating-type locus schizosaccharomyces-pombe dna-replication s-pombe epigenetic control genome-wide c. elegans site heterochromatin
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Martienssen lab
CSHL Cancer Center Program > Gene Regulation and Cell Proliferation
Depositing User: Matt Covey
Date: December 2012
Date Deposited: 13 Dec 2013 16:49
Last Modified: 13 Oct 2015 14:39
PMCID: PMC3909218
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28978

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