Arabidopsis genes essential for seedling viability: Isolation of Insertional mutants and molecular cloning

Budziszewski, G. J., Lewis, S. P., Glover, L. W., Reineke, J., Jones, G., Ziemnik, L. S., Lonowski, J., Nyfeler, B., Aux, G., Zhou, Q., McElver, J., Patton, D. A., Martienssen, R., Grossniklaus, U., Ma, H., Law, M., Levin, J. Z. (December 2001) Arabidopsis genes essential for seedling viability: Isolation of Insertional mutants and molecular cloning. Genetics, 159 (4). pp. 1765-1778. ISSN 0016-6731

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URL: http://www.ncbi.nlm.nih.gov/pubmed/11779813

Abstract

We have undertaken a large-scale genetic screen to identify genes with a seedling-lethal mutant phenotype. From screening similar to38,000 insertional mutant lines, we identified >500 seedling-lethal mutants, completed cosegregation analysis of the insertion and the lethal phenotype for >200 mutants, molecularly characterized 54 mutants, and provided a detailed description for 22 of them. Most of the seedling-lethal mutants seem to affect chloroplast function because they display altered pigmentation and affect genes encoding proteins predicted to have chloroplast localization. Although a high level of functional redundancy in Arabidopsis might be expected because 65% of genes are members of gene families, we found that 41% of the essential genes found in this study are members of Arabidopsis gene families. In addition, we isolated several interesting classes of mutants and genes. We found three mutants in the recently discovered nonmevalonate isoprenoid biosynthetic pathway and mutants disrupting genes similar to Tic40 and tatC, which are likely to be involved in chloroplast protein translocation. Finally, we directly compared T-DNA and Ac/Ds transposon mutagenesis methods in Arabidopsis on a genome scale. In each population, we found only about one-third of the insertion mutations cosegregated with a mutant phenotype.

Item Type: Paper
Uncontrolled Keywords: dependent protein-transport double-stranded-rna isoprenoid biosynthesis chloroplast development caenorhabditis-elegans hypocotyl elongation transcription factor functional genomics signal-transduction reverse genetics
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
organism description > plant
organs, tissues, organelles, cell types and functions > tissues types and functions > transport > protein transport
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date: December 2001
Date Deposited: 13 Dec 2013 16:55
Last Modified: 13 Dec 2013 16:55
PMCID: PMC1461917
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28966

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