Transposon mutagenesis in the study of plant development

May, B. P., Martienssen, R. A. (2003) Transposon mutagenesis in the study of plant development. Critical Reviews in Plant Sciences, 22 (1). pp. 1-35. ISSN 0735-2689

URL: http://www.tandfonline.com/doi/abs/10.1080/7136108...
DOI: 10.1080/713610849

Abstract

Transposon mutagenesis has provided one of the first and most important routes to gene identification and characterization. In the 17 years since the bz1 gene was first tagged with Activator (Ac), more than 60 genes involved in plant development have been cloned using elements such as Supressor-mutator (Spm) and Mutator (Mu) from maize and Tag1 from Arabidopsis. The advantages of transposon-induced alleles in the study of developmental processes go beyond cloning to include sector analysis, generation of new alleles, and conditional expression based on suppression. The laborious technique of directed tagging that led to many of these successes is now being supplanted by systematic projects to produce large collections of transposon insertions that are precharacterized using PCR-based methods and publicly accessible for both forward and reverse genetics. Of the tens of thousands of new genes postulated to exist in Arabidopsis and other species, most are turning out to have no obvious phenotypic effect. The challenge for functional genomics is now to elucidate the apparently subtle actions of genes at a rate commensurate with their discovery.

Item Type: Paper
Uncontrolled Keywords: maize homeobox gene regional insertional mutagenesis controlling element activator tobacco retrotransposon tto1 asymmetric interlaced pcr cell lineage patterns cdna scanning method polycomb-group gene zea-mays contains r2r3 myb genes
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations > mutagenesis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date: 2003
Date Deposited: 27 Mar 2013 15:33
Last Modified: 27 Mar 2013 15:33
URI: http://repository.cshl.edu/id/eprint/28035

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