Three PIGGYBACK genes that specifically influence leaf patterning encode ribosomal proteins

Pinon, V., Etchells, J. P., Rossignol, P., Collier, S. A., Arroyo, J. M., Martienssen, R. A., Byrne, M. E. (April 2008) Three PIGGYBACK genes that specifically influence leaf patterning encode ribosomal proteins. Development, 135 (7). pp. 1315-24. ISSN 0950-1991 (Print)0950-1991 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/18305008
DOI: 10.1242/dev.016469

Abstract

Leaves are determinate organs that arise from the flanks of the shoot apical meristem as polar structures with distinct adaxial (dorsal) and abaxial (ventral) sides. Opposing regulatory interactions between genes specifying adaxial or abaxial fates function to maintain dorsoventral polarity. One component of this regulatory network is the Myb-domain transcription factor gene ASYMMETRIC LEAVES1 (AS1). The contribution of AS1 to leaf polarity varies across different plant species; however, in Arabidopsis, as1 mutants have only mild defects in leaf polarity, suggesting that alternate pathways exist for leaf patterning. Here, we describe three genes, PIGGYBACK1 (PGY1), PGY2 and PGY3, which alter leaf patterning in the absence of AS1. All three pgy mutants develop dramatic ectopic lamina outgrowths on the adaxial side of the leaf in an as1 mutant background. This leaf-patterning defect is enhanced by mutations in the adaxial HD-ZIPIII gene REVOLUTA (REV), and is suppressed by mutations in abaxial KANADI genes. Thus, PGY genes influence leaf development via genetic interactions with the HD-ZIPIII-KANADI pathway. PGY1, PGY2 and PGY3 encode cytoplasmic large subunit ribosomal proteins, L10a, L9 and L5, respectively. Our results suggest a role for translation in leaf dorsoventral patterning and indicate that ribosomes are regulators of key patterning events in plant development.

Item Type: Paper
Uncontrolled Keywords: Alleles Arabidopsis genetics growth & development physiology Arabidopsis Proteins genetics physiology Body Patterning Gene Expression Regulation Plant Genes Plant Homeodomain Proteins genetics physiology In Situ Hybridization Plant Leaves growth & development ultrastructure Point Mutation Ribosomal Proteins physiology Transcription Factors genetics physiology
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > plant
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date: April 2008
Date Deposited: 25 Feb 2013 15:06
Last Modified: 25 Feb 2013 15:06
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27633

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