Mutants of H-ras that interfere with RAS effector function in Saccharomyces cerevisiae

Michaeli, T., Field, J., Ballester, R., O'Neill, K., Wigler, M. H. (October 1989) Mutants of H-ras that interfere with RAS effector function in Saccharomyces cerevisiae. Embo J, 8 (10). pp. 3039-44. ISSN 0261-4189

URL: https://www.ncbi.nlm.nih.gov/pubmed/2684634

Abstract

We report a class of interfering mutants of the human H-ras gene capable of inhibiting phenotypes arising from the expression of the activated RAS2 gene, RAS2val19, in the yeast Saccharomyces cerevisiae. All these mutants encode unprocessed H-ras proteins that remain in the cytoplasm. One of the mutants, H-rasarg186, was examined in detail. H-rasarg186 protein is a competitive inhibitor of RAS2val19 protein. It does not interfere with processing and membrane localization of RAS2val19, nor does it appear to compete with RAS protein for its proposed regulator, the CDC25 protein. By several criteria the RAS2val19 adenylate cyclase interaction is unaffected by H-rasarg186. We infer from our results that H-rasarg186 protein interferes with an alternative function of RAS2val19.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Adenylate Cyclase genetics metabolism Binding Competitive Cell Membrane metabolism Codon genetics Cytoplasm metabolism Fungal Proteins genetics metabolism Gene Expression Regulation, Fungal Genes ras Humans Mutation Phenotype Plasmids Saccharomyces cerevisiae genetics/metabolism Saccharomyces cerevisiae Proteins ras Proteins
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
CSHL Authors:
Communities: CSHL labs > Wigler lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: October 1989
Date Deposited: 12 Apr 2012 13:44
Last Modified: 07 Jan 2019 19:47
PMCID: PMC401381
URI: http://repository.cshl.edu/id/eprint/26231

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