Expression of three mammalian cDNAs that interfere with RAS function in Saccharomyces cerevisiae

Colicelli, J., Nicolette, C., Birchmeier, C., Rodgers, L., Riggs, M., Wigler, M. H. (April 1991) Expression of three mammalian cDNAs that interfere with RAS function in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A, 88 (7). pp. 2913-7. ISSN 0027-8424

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URL: https://www.ncbi.nlm.nih.gov/pubmed/1849280
DOI: 10.1073/pnas.88.7.2913

Abstract

Saccharomyces cerevisiae strains expressing the activated RAS2Val19 gene or lacking both cAMP phosphodiesterase genes, PDE1 and PDE2, have impaired growth control and display an acute sensitivity to heat shock. We have isolated two classes of mammalian cDNAs from yeast expression libraries that suppress the heat shock-sensitive phenotype of RAS2Val19 strain. Members of the first class of cDNAs also suppress the heat shock-sensitive phenotype of pde1- pde2- strains and encode cAMP phosphodiesterases. Members of the second class fail to suppress the phenotype of pde1- pde2- strains and therefore are candidate cDNAs encoding proteins that interact with RAS proteins. We report the nucleotide sequence of three members of this class. Two of these cDNAs share considerable sequence similarity, but none are clearly similar to previously isolated genes.

Item Type: Paper
Uncontrolled Keywords: 3',5'-Cyclic-Nucleotide Phosphodiesterase genetics Amino Acid Sequence Base Sequence Cloning, Molecular Comparative Study DNA genetics Escherichia coli genetics Fungal Proteins genetics GTP-Binding Proteins genetics Gene Library Genes Fungal Genes Suppressor Genes ras Humans Molecular Sequence Data Oligonucleotide Probes Plasmids Restriction Mapping Saccharomyces cerevisiae genetics Sequence Homology Nucleic Acid ras Proteins
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 1 April 1991
Date Deposited: 19 Apr 2012 14:32
Last Modified: 09 Nov 2017 20:20
PMCID: PMC51350
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26173

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