Rescue of a Drosophila NF1 mutant phenotype by protein kinase A

The, I., Hannigan, G. E., Cowley, G. S., Reginald, S., Zhong, Y., Gusella, J. F., Hariharan, I. K., Bernards, A. (1997) Rescue of a Drosophila NF1 mutant phenotype by protein kinase A. Science, 276 (5313). pp. 791-794. ISSN 00368075 (ISSN)

URL: http://www.scopus.com/record/display.url?eid=2-s2....
DOI: 10.1126/science.276.5313.791

Abstract

The neurofibromatosis type 1 (NF1) tumor suppressor protein is thought to restrict cell proliferation by functioning as a Ras-specific guanosine triphosphatase-activating protein. However, Drosophila homozygous for null mutations of an NF1 homolog showed no obvious signs of perturbed Ras1- mediated signaling. Loss of NF1 resulted in a reduction in size of larvae, pupae, and adults. This size defect was not modified by manipulating Ras1 signaling but was restored by expression of activated adenosine 3',5'- monophosphate-dependent protein kinase (PKA). Thus, NF1 and PKA appear to interact in a pathway that controls the overall growth of Drosophila.

Item Type: Paper
Additional Information: PubMed ID: 9115203
Uncontrolled Keywords: cyclic amp dependent protein kinase guanosine triphosphatase activating protein ras protein allele article cell proliferation drosophila gene mutation neurofibromatosis nonhuman oncogene ras phenotype priority journal tumor suppressor gene Amino Acid Sequence Animals Cell Count Cyclic AMP Cyclic AMP-Dependent Protein Kinases Drosophila Proteins Genes, Insect GTP Phosphohydrolases Insect Proteins Molecular Sequence Data Mutation Nerve Tissue Proteins Neurofibromin 1 Proteins ras GTPase-Activating Proteins ras Proteins Recombinant Fusion Proteins Signal Transduction
Subjects: organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > NF1
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
CSHL Authors:
Communities: CSHL labs > Zhong lab
Depositing User: CSHL Librarian
Date: 1997
Date Deposited: 29 Mar 2012 20:30
Last Modified: 29 Mar 2012 20:30
URI: http://repository.cshl.edu/id/eprint/25579

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