The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation

Lavy, T., Kumar, P. R., He, H. Z., Joshua-Tor, L. (2012) The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation. Genes & Development, 26 (3). pp. 294-303. ISSN 0890-9369

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URL: http://www.ncbi.nlm.nih.gov/pubmed/22302941
DOI: 10.1101/gad.182691.111

Abstract

A wealth of genetic information and some biochemical analysis have made the GAL regulon of the yeast Saccharomyces cerevisiae a classic model system for studying transcriptional activation in eukaryotes. Galactose induces this transcriptional switch, which is regulated by three proteins: the transcriptional activator Gal4p, bound to DNA; the repressor Gal80p; and the transducer Gal3p. We showed previously that NADP appears to act as a trigger to kick the repressor off the activator. Sustained activation involves a complex of the transducer Gal3p and Gal80p mediated by galactose and ATP. We solved the crystal structure of the complex of Gal3p-Gal80p with alpha-D-galactose and ATP to 2.1 angstrom resolution. The interaction between the proteins occurs only when Gal3p is in a "closed" state induced by ligand binding. The structure of the complex provides a rationale for the phenotypes of several well-known Gal80p and Gal3p mutants as well as the lack of galactokinase activity of Gal3p.

Item Type: Paper
Uncontrolled Keywords: transcription regulation activation structural biology transcriptional repression dna-binding domain saccharomyces-cerevisiae molecular-structure transcriptional activator gal4p-activated genes substrate-specificity amino-acids protein galactokinase galactose
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein structure rendering
Investigative techniques and equipment > x ray crystallography
CSHL Authors:
Communities: CSHL Cancer Center Program > Gene Regulation and Cell Proliferation
CSHL Cancer Center Shared Resources > Proteomics Service
CSHL Post Doctoral Fellows
CSHL labs > Joshua-Tor lab
CSHL Cancer Center Shared Resources > Mass Spectrometry Service
Depositing User: CSHL Librarian
Date Deposited: 22 Mar 2012 14:24
Last Modified: 30 Oct 2015 14:30
PMCID: PMC3278896
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25463

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