Sequencing the maize genome

Martienssen, R. A., Rabinowicz, P. D., O'Shaughnessy, A., McCombie, W. R. (April 2004) Sequencing the maize genome. Curr Opin Plant Biol, 7 (2). pp. 102-7. ISSN 1369-5266 (Print)

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URL: http://www.scopus.com/record/display.url?eid=2-s2....
DOI: 10.1016/j.pbi.2004.01.010

Abstract

Sequencing of complex genomes can be accomplished by enriching shotgun libraries for genes. In maize, gene-enrichment by copy-number normalization (high C(0)t) and methylation filtration (MF) have been used to generate up to two-fold coverage of the gene-space with less than 1 million sequencing reads. Simulations using sequenced bacterial artificial chromosome (BAC) clones predict that 5x coverage of gene-rich regions, accompanied by less than 1x coverage of subclones from BAC contigs, will generate high-quality mapped sequence that meets the needs of geneticists while accommodating unusually high levels of structural polymorphism. By sequencing several inbred strains, we propose a strategy for capturing this polymorphism to investigate hybrid vigor or heterosis.

Item Type: Paper
Uncontrolled Keywords: Chromosomes Artificial Bacterial genetics Genome Plant Physical Chromosome Mapping methods Zea mays genetics ZEA Zea
Subjects: organism description > plant > maize
Communities: CSHL labs > Martienssen lab
CSHL labs > McCombie lab
Depositing User: CSHL Librarian
Date: April 2004
Date Deposited: 30 Jan 2012 20:28
Last Modified: 30 Jan 2012 20:28
URI: http://repository.cshl.edu/id/eprint/22436

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