Martienssen, R. A., Rabinowicz, P. D., O'Shaughnessy, A., McCombie, W. R. (April 2004) Sequencing the maize genome. Curr Opin Plant Biol, 7 (2). pp. 102-7. ISSN 1369-5266 (Print)
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Abstract
Sequencing of complex genomes can be accomplished by enriching shotgun libraries for genes. In maize, gene-enrichment by copy-number normalization (high C(0)t) and methylation filtration (MF) have been used to generate up to two-fold coverage of the gene-space with less than 1 million sequencing reads. Simulations using sequenced bacterial artificial chromosome (BAC) clones predict that 5x coverage of gene-rich regions, accompanied by less than 1x coverage of subclones from BAC contigs, will generate high-quality mapped sequence that meets the needs of geneticists while accommodating unusually high levels of structural polymorphism. By sequencing several inbred strains, we propose a strategy for capturing this polymorphism to investigate hybrid vigor or heterosis.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Chromosomes Artificial Bacterial genetics Genome Plant Physical Chromosome Mapping methods Zea mays genetics ZEA Zea |
| Subjects: | organism description > plant > maize |
| Communities: | CSHL labs > Martienssen lab CSHL labs > McCombie lab |
| Depositing User: | CSHL Librarian |
| Date: | April 2004 |
| Date Deposited: | 30 Jan 2012 20:28 |
| Last Modified: | 30 Jan 2012 20:28 |
| URI: | https://repository.cshl.edu/id/eprint/22436 |
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