Qi, Xiaoli, Muñoz-Castañeda, Rodrigo, Yue, Yuanlei, Xiao, Sheng, Ding, Liya, Narasimhan, Arun, Chen, Xin, Wang, Wei, Li, Yuanyuan, Elowsky, Corey, Palmer, Jason, Drewes, Rhonda, Sun, Jianjun, Wu, Jinglei, Qu, Lei, Wu, Zhuhao, Peng, Hanchuan, Osten, Pavel (November 2025) Confocal Airy beam oblique light-sheet tomography for brain-wide cell type distribution and morphology. Nature Methods. ISSN 1548-7091
Abstract
Advanced brain-wide mapping is critical for addressing complex questions in neuroscience. However, current imaging methods are limited by throughput, resolution and signal-to-noise ratio, constraining their broader applicability. Here, we present confocal Airy beam integrated with single-photon oblique light-sheet tomography (CAB-OLST): a system that integrates single-photon excitation with a scanned Airy beam light sheet, virtual slit detection and automated mechanical sectioning. CAB-OLST enables high-throughput, high-resolution and high-signal-to-noise ratio volumetric imaging, achieving an optical resolution of 0.77 μm × 0.49 μm × 2.61 μm. This allows for mouse brain-wide cell type distribution mapping at a voxel size of 0.37 μm × 0.37 μm × 1.77 μm in 10 h and single-neuron projectome imaging with a voxel size of 0.26 μm × 0.26 μm × 1.06 μm over 58 h. Compared to existing light-sheet and point-scanning systems, CAB-OLST provides a scalable and robust platform for comprehensive neuronal morphology reconstruction and high-precision cell atlas generation.
| Item Type: | Paper |
|---|---|
| Subjects: | neurobiology neurobiology > neuroscience |
| CSHL Authors: | |
| Communities: | CSHL labs > Mitra lab CSHL labs > Osten lab CSHL labs > Trotman lab CSHL Post Doctoral Fellows |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 13 November 2025 |
| Date Deposited: | 17 Nov 2025 14:06 |
| Last Modified: | 17 Nov 2025 14:06 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/42006 |
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