Boyd, Leah, Berisha, Adrian, Gomez, Adrian M, Gibson, Erin M, Borniger, Jeremy C (July 2025) Enduring non-rapid eye movement sleep fragmentation following methotrexate chemotherapy in cancer-naïve mice. Sleep, 48 (7). zsaf073. ISSN 0161-8105
Abstract
STUDY OBJECTIVES: Sleep disruption is common in people with cancer and survivors, but understanding the mechanisms driving these problems is difficult due to heterogeneity among cancers, patients, and treatment modalities. We investigated whether the common antifolate chemotherapeutic agent methotrexate (MTX) promotes changes in sleep independent of cancer in adult mice. METHODS: Adult mice (>7 weeks old, both sexes, n = 13) were exposed to either a clinically relevant chemotherapy regimen with methotrexate (n = 7) or saline (control, n = 6) accompanied by continuous EEG/EMG telemetry recording. Sleep states were scored as either wake, non-rapid eye movement (NREM) sleep, or REM sleep in 5-second epochs weekly during MTX or saline treatment and then 2 weeks following the last injection to examine enduring changes in sleep-wake cycles. RESULTS: MTX exposure caused NREM sleep fragmentation, indicated by (1) shorter and more frequent NREM sleep bouts, (2) more transitions between wake and NREM sleep, and (3) more accumulated NREM sleep bouts over time. These effects were first detected after the second MTX injection and lasted into the 2-week follow-up recording. MTX did not alter delta power in NREM sleep, indicating no changes to sleep quality. The total time spent in each vigilance state remained unaffected by MTX use. Finally, when given MTX, male mice displayed more fragmented sleep compared to female mice. CONCLUSIONS: Methotrexate promotes NREM sleep fragmentation, without affecting sleep quality or time spent asleep. This effect is stronger in males. These data suggest that chemotherapy can cause long-term sleep disruption independent of cancer presence.
| Item Type: | Paper |
|---|---|
| Subjects: | diseases & disorders > cancer diseases & disorders organism description > animal behavior > REM sleep organism description > animal organism description > animal behavior organism description > animal > mammal organism description > animal > mammal > rodent > mouse organism description > animal > mammal > rodent |
| CSHL Authors: | |
| Communities: | CSHL labs > Borniger lab CSHL Cancer Center Program CSHL Cancer Center Program > Cellular Communication in Cancer Program |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 11 July 2025 |
| Date Deposited: | 23 Jul 2025 14:04 |
| Last Modified: | 23 Jul 2025 14:04 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/41915 |
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