Heterologous Expression and Purification of SARS-CoV2 Nucleocapsid Protein

Garg, Ankur, Liu, Lihong, Ho, David, Joshua-Tor, Leemor (2020) Heterologous Expression and Purification of SARS-CoV2 Nucleocapsid Protein. Bio-protocol, 10 (8). ISSN 2331-8325

Abstract

This protocol describes a step by step method for heterologous expression of SARS-CoV2 Nucleocapsid (N) protein in Escherichia coli. Moreover, this protocol includes steps to purify the N protein to high purity and homogeneity. Thus, purified protein can be used for ligand binding assays and other biochemical experiments.

Item Type: Paper
Subjects: diseases & disorders
diseases & disorders > viral diseases
diseases & disorders > viral diseases > coronavirus
diseases & disorders > viral diseases > coronavirus > covid 19
CSHL Authors:
Communities: CSHL labs > Joshua-Tor lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 2020
Date Deposited: 16 Oct 2024 13:52
Last Modified: 16 Oct 2024 13:52
Related URLs:
URI: https://repository.cshl.edu/id/eprint/41708

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