Shen, Xiaomeng, Liu, Lei, Tran, Thu, Ning, Qiang, Li, Manfei, Huang, Liangliang, Zhao, Ran, Li, Yunfu, Qing, Xiangyu, Jackson, David, Bai, Yan, Song, Weibin, Lai, Jinsheng, Zhang, Zuxin, Zhao, Haiming (September 2024) KRN5b regulates maize kernel row number through mediating phosphoinositol signalling. Plant Biotechnology Journal. ISSN 1467-7652 (Public Dataset)
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Abstract
Kernel row number (KRN) is a major yield related trait for maize (Zea mays L.) and is also a major goal of breeders, as it can increase the number of kernels per plant. Thus, identifying new genetic factors involving in KRN formation may accelerate improving yield-related traits genetically. We herein describe a new kernel number-related gene (KRN5b) identified from KRN QTL qKRN5b and encoding an inositol polyphosphate 5-phosphatase (5PTase). KRN5b has phosphatase activity towards PI(4,5)P2, PI(3,4,5)P3, and Ins(1,4,5)P3 in vitro. Knocking out KRN5b caused accumulation of PI(4,5)P2 and Ins(1,4,5)P3, resulting in disordered kernel rows and a decrease in the number of kernels and tassel branches. The introgression of the allele with higher expression abundance into different inbred lines could increase the ear weight of the inbred lines and the corresponding hybrids by 10.1%-12.2% via increasing KRN, with no adverse effects on other agronomic traits. Further analyses showed that KRN5b regulates inflorescence development through affecting the synthesis and distribution of hormones. Together, KRN5b contributes to spikelet pair meristem development through inositol phosphate and phosphatidylinositols, making it a selecting target for yield improvement.
| Item Type: | Paper |
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| Subjects: | organism description > plant > maize organism description > plant |
| CSHL Authors: | |
| Communities: | CSHL labs > Jackson lab |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 20 September 2024 |
| Date Deposited: | 23 Sep 2024 13:33 |
| Last Modified: | 23 Sep 2024 13:33 |
| Dataset ID: |
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| URI: | https://repository.cshl.edu/id/eprint/41681 |
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