Pseudouridine guides germline small RNA transport and epigenetic inheritance

Herridge, Rowan P, Dolata, Jakub, Migliori, Valentina, de Santis Alves, Cristiane, Borges, Filipe, Schorn, Andrea J, van Ex, Frédéric, Lin, Ann, Bajczyk, Mateusz, Parent, Jean-Sebastien, Leonardi, Tommaso, Hendrick, Alan, Kouzarides, Tony, Martienssen, Robert A (September 2024) Pseudouridine guides germline small RNA transport and epigenetic inheritance. Nature Structural & Molecular Biology. ISSN 1545-9993 (Public Dataset)

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Abstract

Developmental epigenetic modifications in plants and animals are mostly reset during gamete formation but some are inherited from the germline. Small RNAs guide these epigenetic modifications but how inherited small RNAs are distinguished in plants and animals is unknown. Pseudouridine (Ψ) is the most abundant RNA modification but has not been explored in small RNAs. Here, we develop assays to detect Ψ in short RNA sequences, demonstrating its presence in mouse and Arabidopsis microRNAs. Germline small RNAs, namely epigenetically activated small interfering RNAs (easiRNAs) in Arabidopsis pollen and Piwi-interacting RNAs in mouse testes, are enriched for Ψ. In pollen, pseudouridylated easiRNAs are transported to sperm cells from the vegetative nucleus, and PAUSED/HEN5 (PSD), the plant homolog of Exportin-t, interacts genetically with Ψ and is required for this transport. We further show that Exportin-t is required for the triploid block: small RNA dosage-dependent seed lethality that is epigenetically inherited from pollen. Thus, Ψ has a conserved role in marking inherited small RNAs in the germline.

Item Type: Paper
Subjects: organism description > plant > Arabidopsis
bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > epigenetics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > epigenetics
organism description > plant
CSHL Authors:
Communities: CSHL Cancer Center Program > Gene Regulation and Inheritance Program
CSHL labs > Martienssen lab
CSHL labs > Schorn lab
CSHL Cancer Center Program
CSHL Cancer Center Shared Resources
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 6 September 2024
Date Deposited: 09 Sep 2024 13:05
Last Modified: 09 Sep 2024 13:05
Related URLs:
Dataset ID:
URI: https://repository.cshl.edu/id/eprint/41652

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