Tierney, Braden T, Kim, JangKeun, Overbey, Eliah G, Ryon, Krista A, Foox, Jonathan, Sierra, Maria A, Bhattacharya, Chandrima, Damle, Namita, Najjar, Deena, Park, Jiwoon, Garcia Medina, J Sebastian, Houerbi, Nadia, Meydan, Cem, Wain Hirschberg, Jeremy, Qiu, Jake, Kleinman, Ashley S, Al-Ghalith, Gabriel A, MacKay, Matthew, Afshin, Evan E, Dhir, Raja, Borg, Joseph, Gatt, Christine, Brereton, Nicholas, Readhead, Benjamin P, Beyaz, Semir, Venkateswaran, Kasthuri J, Wiseman, Kelly, Moreno, Juan, Boddicker, Andrew M, Zhao, Junhua, Lajoie, Bryan R, Scott, Ryan T, Altomare, Andrew, Kruglyak, Semyon, Levy, Shawn, Church, George M, Mason, Christopher E (June 2024) Longitudinal multi-omics analysis of host microbiome architecture and immune responses during short-term spaceflight. Nature Microbiology. ISSN 2058-5276
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Abstract
Maintenance of astronaut health during spaceflight will require monitoring and potentially modulating their microbiomes. However, documenting microbial shifts during spaceflight has been difficult due to mission constraints that lead to limited sampling and profiling. Here we executed a six-month longitudinal study to quantify the high-resolution human microbiome response to three days in orbit for four individuals. Using paired metagenomics and metatranscriptomics alongside single-nuclei immune cell profiling, we characterized time-dependent, multikingdom microbiome changes across 750 samples and 10 body sites before, during and after spaceflight at eight timepoints. We found that most alterations were transient across body sites; for example, viruses increased in skin sites mostly during flight. However, longer-term shifts were observed in the oral microbiome, including increased plaque-associated bacteria (for example, Fusobacteriota), which correlated with immune cell gene expression. Further, microbial genes associated with phage activity, toxin-antitoxin systems and stress response were enriched across multiple body sites. In total, this study reveals in-depth characterization of microbiome and immune response shifts experienced by astronauts during short-term spaceflight and the associated changes to the living environment, which can help guide future missions, spacecraft design and space habitat planning.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes |
| CSHL Authors: | |
| Communities: | CSHL labs > Beyaz lab |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 11 June 2024 |
| Date Deposited: | 17 Jun 2024 13:04 |
| Last Modified: | 01 Oct 2024 17:53 |
| PMCID: | PMC11222149 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/41583 |
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