Light and temperature regulate m6A-RNA modification to regulate growth in plants

Artz, Oliver, Ackermann, Amanda, Taylor, Laura, Koo, Peter K, Pedmale, Ullas V (January 2023) Light and temperature regulate m6A-RNA modification to regulate growth in plants. bioRxiv. (Submitted)

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Abstract

N6-methyladenosine is a highly dynamic, abundant mRNA modification which is an excellent potential mechanism for fine tuning gene expression. Plants adapt to their surrounding light and temperature environment using complex gene regulatory networks. The role of m6A in controlling gene expression in response to variable environmental conditions has so far been unexplored. Here, we map the transcriptome-wide m6A landscape under various light and temperature environments. Identified m6A-modifications show a highly specific spatial distribution along transcripts with enrichment occurring in 5’UTR regions and around transcriptional end sites. We show that the position of m6A modifications on transcripts might influence cellular transcript localization and the presence of m6A-modifications is associated with alternative polyadenylation, a process which results in multiple RNA isoforms with varying 3’UTR lengths. RNA with m6A-modifications exhibit a higher preference for shorter 3’UTRs. These shorter 3’UTR regions might directly influence transcript abundance and localization by including or excluding cis-regulatory elements. We propose that environmental stimuli might change the m6A landscape of plants as one possible way of fine tuning gene regulation through alternative polyadenylation and transcript localization.

Item Type:	Paper
Subjects:	bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing organism description > plant
CSHL Authors:	Koo, Peter K Pedmale, Vincent Ackermann, Amanda Artz, Oliver Taylor, Laura
Communities:	CSHL labs > Koo Lab CSHL labs > Pedmale lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	17 January 2023
Date Deposited:	31 Oct 2023 19:49
Last Modified:	20 Jun 2024 18:02
PMCID:	PMC9882139
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/41285

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