Transcriptomic cytoarchitecture reveals principles of human neocortex organization

Jorstad, Nikolas L, Close, Jennie, Johansen, Nelson, Yanny, Anna Marie, Barkan, Eliza R, Travaglini, Kyle J, Bertagnolli, Darren, Campos, Jazmin, Casper, Tamara, Crichton, Kirsten, Dee, Nick, Ding, Song-Lin, Gelfand, Emily, Goldy, Jeff, Hirschstein, Daniel, Kiick, Katelyn, Kroll, Matthew, Kunst, Michael, Lathia, Kanan, Long, Brian, Martin, Naomi, McMillen, Delissa, Pham, Trangthanh, Rimorin, Christine, Ruiz, Augustin, Shapovalova, Nadiya, Shehata, Soraya, Siletti, Kimberly, Somasundaram, Saroja, Sulc, Josef, Tieu, Michael, Torkelson, Amy, Tung, Herman, Callaway, Edward M, Hof, Patrick R, Keene, C Dirk, Levi, Boaz P, Linnarsson, Sten, Mitra, Partha P, Smith, Kimberly, Hodge, Rebecca D, Bakken, Trygve E, Lein, Ed S (October 2023) Transcriptomic cytoarchitecture reveals principles of human neocortex organization. Science, 382 (6667). eadf6812. ISSN 0036-8075

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URL: https://www.ncbi.nlm.nih.gov/pubmed/37824655

DOI: 10.1126/science.adf6812

Abstract

Variation in cytoarchitecture is the basis for the histological definition of cortical areas. We used single cell transcriptomics and performed cellular characterization of the human cortex to better understand cortical areal specialization. Single-nucleus RNA-sequencing of 8 areas spanning cortical structural variation showed a highly consistent cellular makeup for 24 cell subclasses. However, proportions of excitatory neuron subclasses varied substantially, likely reflecting differences in connectivity across primary sensorimotor and association cortices. Laminar organization of astrocytes and oligodendrocytes also differed across areas. Primary visual cortex showed characteristic organization with major changes in the excitatory to inhibitory neuron ratio, expansion of layer 4 excitatory neurons, and specialized inhibitory neurons. These results lay the groundwork for a refined cellular and molecular characterization of human cortical cytoarchitecture and areal specialization.

Item Type:	Paper
Subjects:	bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics organism description > animal organism description > animal > mammal > primates > hominids organism description > animal > mammal > primates > hominids > human organism description > animal > mammal organs, tissues, organelles, cell types and functions > tissues types and functions > neocortex organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions organism description > animal > mammal > primates organs, tissues, organelles, cell types and functions > tissues types and functions bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transcriptomes
CSHL Authors:	Mitra, Partha P.
Communities:	CSHL labs > Mitra lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	13 October 2023
Date Deposited:	20 Oct 2023 13:14
Last Modified:	10 Jan 2024 19:42
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/41277

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