Manzi, Lucio, Barrow, Andrew S, Hopper, Jonathan TS, Kaminska, Renata, Kleanthous, Colin, Robinson, Carol V, Moses, John E, Oldham, Neil J (November 2017) Carbene Footprinting Reveals Binding Interfaces of a Multimeric Membrane‐Spanning Protein. Angewandte Chemie International Edition, 129 (47). pp. 15069-15073. ISSN 0044-8249
Abstract
Mapping the interaction sites between membrane‐spanning proteins is a key challenge in structural biology. In this study a carbene‐footprinting approach was developed and applied to identify the interfacial sites of a trimeric, integral membrane protein, OmpF, solubilised in micelles. The diazirine‐based footprinting probe is effectively sequestered by, and incorporated into, the micelles, thus leading to efficient labelling of the membrane‐spanning regions of the protein upon irradiation at 349 nm. Areas associated with protein–protein interactions between the trimer subunits remained unlabelled, thus revealing their location.
| Item Type: | Paper |
|---|---|
| Subjects: | organs, tissues, organelles, cell types and functions > tissues types and functions > transport > membrane transport |
| CSHL Authors: | |
| Communities: | CSHL labs > Moses lab |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 20 November 2017 |
| Date Deposited: | 03 Oct 2023 20:49 |
| Last Modified: | 03 Oct 2023 20:49 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/41120 |
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