An adaptive teosinte mexicana introgression modulates phosphatidylcholine levels and is associated with maize flowering time

Barnes, Allison C, Rodríguez-Zapata, Fausto, Juárez-Núñez, Karla A, Gates, Daniel J, Janzen, Garrett M, Kur, Andi, Wang, Li, Jensen, Sarah E, Estévez-Palmas, Juan M, Crow, Taylor M, Kavi, Heli S, Pil, Hannah D, Stokes, Ruthie L, Knizner, Kevan T, Aguilar-Rangel, Maria R, Demesa-Arévalo, Edgar, Skopelitis, Tara, Pérez-Limón, Sergio, Stutts, Whitney L, Thompson, Peter, Chiu, Yu-Chun, Jackson, David, Muddiman, David C, Fiehn, Oliver, Runcie, Daniel, Buckler, Edward S, Ross-Ibarra, Jeffrey, Hufford, Matthew B, Sawers, Ruairidh JH, Rellán-Álvarez, Rubén (July 2022) An adaptive teosinte mexicana introgression modulates phosphatidylcholine levels and is associated with maize flowering time. Proceedings of the National Academy of Sciences of USA, 119 (27). e2100036119. ISSN 0027-8424

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Abstract

Native Americans domesticated maize (Zea mays ssp. mays) from lowland teosinte parviglumis (Zea mays ssp. parviglumis) in the warm Mexican southwest and brought it to the highlands of Mexico and South America where it was exposed to lower temperatures that imposed strong selection on flowering time. Phospholipids are important metabolites in plant responses to low-temperature and phosphorus availability and have been suggested to influence flowering time. Here, we combined linkage mapping with genome scans to identify High PhosphatidylCholine 1 (HPC1), a gene that encodes a phospholipase A1 enzyme, as a major driver of phospholipid variation in highland maize. Common garden experiments demonstrated strong genotype-by-environment interactions associated with variation at HPC1, with the highland HPC1 allele leading to higher fitness in highlands, possibly by hastening flowering. The highland maize HPC1 variant resulted in impaired function of the encoded protein due to a polymorphism in a highly conserved sequence. A meta-analysis across HPC1 orthologs indicated a strong association between the identity of the amino acid at this position and optimal growth in prokaryotes. Mutagenesis of HPC1 via genome editing validated its role in regulating phospholipid metabolism. Finally, we showed that the highland HPC1 allele entered cultivated maize by introgression from the wild highland teosinte Zea mays ssp. mexicana and has been maintained in maize breeding lines from the Northern United States, Canada, and Europe. Thus, HPC1 introgressed from teosinte mexicana underlies a large metabolic QTL that modulates phosphatidylcholine levels and has an adaptive effect at least in part via induction of early flowering time.

Item Type:	Paper
Subjects:	bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > alleles bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > phospholipase A organism description > plant bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:	Skopelitis, Tara Jackson, David P. Demesa Arevalo, Edgar
Communities:	CSHL labs > Jackson lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	5 July 2022
Date Deposited:	05 Jul 2022 22:14
Last Modified:	11 Jan 2024 15:50
PMCID:	PMC9271162
URI:	https://repository.cshl.edu/id/eprint/40672

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