Maiorino, Laura, Shevik, Margaret, Adrover, José M, Han, Xiao, Georgas, Elias, Wilkinson, John Erby, Seidner, Harrison, Foerschner, Leonie, Tuveson, David A, Qin, Yi-Xian, Egeblad, Mikala (January 2022) Longitudinal Intravital Imaging Through Clear Silicone Windows. Journal of Visualized Experiments (179). ISSN 1940-087X
Abstract
Intravital microscopy (IVM) enables visualization of cell movement, division, and death at single-cell resolution. IVM through surgically inserted imaging windows is particularly powerful because it allows longitudinal observation of the same tissue over days to weeks. Typical imaging windows comprise a glass coverslip in a biocompatible metal frame sutured to the mouse's skin. These windows can interfere with the free movement of the mice, elicit a strong inflammatory response, and fail due to broken glass or torn sutures, any of which may necessitate euthanasia. To address these issues, windows for long-term abdominal organ and mammary gland imaging were developed from a thin film of polydimethylsiloxane (PDMS), an optically clear silicone polymer previously used for cranial imaging windows. These windows can be glued directly to the tissues, reducing the time needed for insertion. PDMS is flexible, contributing to its durability in mice over time-up to 35 days have been tested. Longitudinal imaging is imaging of the same tissue region during separate sessions. A stainless-steel grid was embedded within the windows to localize the same region, allowing the visualization of dynamic processes (like mammary gland involution) at the same locations, days apart. This silicone window also allowed monitoring of single disseminated cancer cells developing into micro-metastases over time. The silicone windows used in this study are simpler to insert than metal-framed glass windows and cause limited inflammation of the imaged tissues. Moreover, embedded grids allow for straightforward tracking of the same tissue region in repeated imaging sessions.
| Item Type: | Paper |
|---|---|
| Subjects: | Investigative techniques and equipment organism description > animal Investigative techniques and equipment > imaging Investigative techniques and equipment > imaging > intravital imaging organism description > animal > mammal organism description > animal > mammal > rodent > mouse organism description > animal > mammal > rodent |
| CSHL Authors: | |
| Communities: | CSHL Cancer Center Program CSHL Cancer Center Program > Cellular Communication in Cancer Program CSHL Cancer Center Shared Resources CSHL labs > Egeblad lab CSHL labs > Tuveson lab School of Biological Sciences > Publications |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 5 January 2022 |
| Date Deposited: | 26 Jan 2022 16:24 |
| Last Modified: | 29 Feb 2024 21:00 |
| PMCID: | PMC9286001 |
| URI: | https://repository.cshl.edu/id/eprint/40500 |
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