An RNA exosome subunit mediates cell-to-cell trafficking of a homeobox mRNA via plasmodesmata

Kitagawa, Munenori, Wu, Peipei, Balkunde, Rachappa, Cunniff, Patrick, Jackson, David (January 2022) An RNA exosome subunit mediates cell-to-cell trafficking of a homeobox mRNA via plasmodesmata. Science, 375 (6577). pp. 177-182. ISSN 0036-8075

URL: https://www.ncbi.nlm.nih.gov/pubmed/35025667

DOI: 10.1126/science.abm0840

Abstract

Messenger RNAs (mRNAs) function as mobile signals for cell-to-cell communication in multicellular organisms. The KNOTTED1 (KN1) homeodomain family transcription factors act non–cell autonomously to control stem cell maintenance in plants through cell-to-cell movement of their proteins and mRNAs through plasmodesmata; however, the mechanism of mRNA movement is largely unknown. We show that cell-to-cell movement of a KN1 mRNA requires ribosomal RNA–processing protein 44A (AtRRP44A), a subunit of the RNA exosome that processes or degrades diverse RNAs in eukaryotes. AtRRP44A can interact with plasmodesmata and mediates the cell-to-cell trafficking of KN1 mRNA, and genetic analysis indicates that AtRRP44A is required for the developmental functions of SHOOT MERISTEMLESS, an Arabidopsis KN1 homolog. Our findings suggest that AtRRP44A promotes mRNA trafficking through plasmodesmata to control stem cell–dependent processes in plants.

Item Type:	Paper
Subjects:	organism description > plant > Arabidopsis bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > exosome complex bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > homeodomain protein organs, tissues, organelles, cell types and functions > tissues types and functions > meristem bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA organs, tissues, organelles, cell types and functions organism description > plant behavior > plasmodesmata organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > plasmodesmata bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:	Kitagawa, Munenori Balkunde, Rachappa Jackson, David P. Wu, Peipei Cunniff, Patrick
Communities:	CSHL labs > Jackson lab CSHL labs > Vakoc lab School of Biological Sciences > Publications
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	14 January 2022
Date Deposited:	20 Jan 2022 15:56
Last Modified:	29 Feb 2024 16:56
URI:	https://repository.cshl.edu/id/eprint/40496

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