Ground tissue circuitry regulates organ complexity in maize and Setaria

Ortiz-Ramírez, Carlos, Guillotin, Bruno, Xu, Xiaosa, Rahni, Ramin, Zhang, Sanqiang, Yan, Zhe, Coqueiro Dias Araujo, Poliana, Demesa-Arevalo, Edgar, Lee, Laura, Van Eck, Joyce, Gingeras, Thomas R, Jackson, David, Gallagher, Kimberly L, Birnbaum, Kenneth D (December 2021) Ground tissue circuitry regulates organ complexity in maize and Setaria. Science, 374 (6572). pp. 1247-1252. ISSN 0036-8075

URL: https://www.ncbi.nlm.nih.gov/pubmed/34855479
DOI: 10.1126/science.abj2327

Abstract

Most plant roots have multiple cortex layers that make up the bulk of the organ and play key roles in physiology, such as flood tolerance and symbiosis. However, little is known about the formation of cortical layers outside of the highly reduced anatomy of Arabidopsis. Here, we used single-cell RNA sequencing to rapidly generate a cell-resolution map of the maize root, revealing an alternative configuration of the tissue formative transcription factor SHORT-ROOT (SHR) adjacent to an expanded cortex. We show that maize SHR protein is hypermobile, moving at least eight cell layers into the cortex. Higher-order SHR mutants in both maize and Setaria have reduced numbers of cortical layers, showing that the SHR pathway controls expansion of cortical tissue to elaborate anatomical complexity.

Item Type: Paper
Subjects: organism description > plant > maize
organs, tissues, organelles, cell types and functions > tissues types and functions > root
Investigative techniques and equipment > assays > Single cell sequencing
CSHL Authors:
Communities: CSHL labs > Gingeras lab
CSHL labs > Jackson lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 3 December 2021
Date Deposited: 08 Dec 2021 21:09
Last Modified: 04 Jan 2022 15:12
PMCID: PMC8719420
URI: https://repository.cshl.edu/id/eprint/40448

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