Ground tissue circuitry regulates organ complexity in maize and Setaria

Ortiz-Ramírez, Carlos, Guillotin, Bruno, Xu, Xiaosa, Rahni, Ramin, Zhang, Sanqiang, Yan, Zhe, Coqueiro Dias Araujo, Poliana, Demesa-Arevalo, Edgar, Lee, Laura, Van Eck, Joyce, Gingeras, Thomas R, Jackson, David, Gallagher, Kimberly L, Birnbaum, Kenneth D (December 2021) Ground tissue circuitry regulates organ complexity in maize and Setaria. Science, 374 (6572). pp. 1247-1252. ISSN 0036-8075

URL: https://www.ncbi.nlm.nih.gov/pubmed/34855479

DOI: 10.1126/science.abj2327

Abstract

Most plant roots have multiple cortex layers that make up the bulk of the organ and play key roles in physiology, such as flood tolerance and symbiosis. However, little is known about the formation of cortical layers outside of the highly reduced anatomy of Arabidopsis. Here, we used single-cell RNA sequencing to rapidly generate a cell-resolution map of the maize root, revealing an alternative configuration of the tissue formative transcription factor SHORT-ROOT (SHR) adjacent to an expanded cortex. We show that maize SHR protein is hypermobile, moving at least eight cell layers into the cortex. Higher-order SHR mutants in both maize and Setaria have reduced numbers of cortical layers, showing that the SHR pathway controls expansion of cortical tissue to elaborate anatomical complexity.

Item Type:	Paper
Subjects:	bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics Investigative techniques and equipment organism description > plant > maize bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification Investigative techniques and equipment > assays Investigative techniques and equipment > flow cytometry organism description > plant bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > plant proteins bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types Investigative techniques and equipment > assays > RNA-seq organs, tissues, organelles, cell types and functions > tissues types and functions > root Investigative techniques and equipment > assays > Single cell sequencing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:	Xu, Xiaosa Gingeras, Thomas R. Jackson, David P. Demesa Arevalo, Edgar
Communities:	CSHL labs > Gingeras lab CSHL labs > Jackson lab CSHL Cancer Center Program CSHL Cancer Center Program > Gene Regulation and Inheritance Program
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	3 December 2021
Date Deposited:	08 Dec 2021 21:09
Last Modified:	13 Feb 2024 19:11
PMCID:	PMC8719420
URI:	https://repository.cshl.edu/id/eprint/40448

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