Automated assembly scaffolding elevates a new tomato system for high-throughput genome editing

Alonge, Michael, Lebeigle, Ludivine, Kirsche, Melanie, Aganezov, Sergey, Wang, Xingang, Lippman, Zachary, Schatz, Michael, Soyk, Sebastian (November 2021) Automated assembly scaffolding elevates a new tomato system for high-throughput genome editing. bioRxiv. (Unpublished)

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Abstract

Advancing crop genomics requires efficient genetic systems enabled by high-quality personalized genome assemblies. Here, we introduce RagTag, a toolset for automating assembly scaffolding and patching, and we establish chromosome-scale reference genomes for the widely used tomato genotype M82 along with Sweet-100, a rapid-cycling genotype that we developed to accelerate functional genomics and genome editing. This work outlines strategies to rapidly expand genetic systems and genomic resources in other plant species.

Item Type:	Paper
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes organism description > plant > tomato
CSHL Authors:	Wang, Xingang Lippman, Zachary B. Schatz, Michael C.
Communities:	CSHL labs > Lippman lab CSHL labs > Schatz lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	19 November 2021
Date Deposited:	07 Dec 2021 19:59
Last Modified:	16 May 2024 14:30
URI:	https://repository.cshl.edu/id/eprint/40446

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