The genetic and epigenetic landscape of the Arabidopsis centromeres

Naish, Matthew, Alonge, Michael, Wlodzimierz, Piotr, Tock, Andrew J, Abramson, Bradley W, Schmücker, Anna, Mandáková, Terezie, Jamge, Bhagyshree, Lambing, Christophe, Kuo, Pallas, Yelina, Natasha, Hartwick, Nolan, Colt, Kelly, Smith, Lisa M, Ton, Jurriaan, Kakutani, Tetsuji, Martienssen, Robert A, Schneeberger, Korbinian, Lysak, Martin A, Berger, Frédéric, Bousios, Alexandros, Michael, Todd P, Schatz, Michael C, Henderson, Ian R (November 2021) The genetic and epigenetic landscape of the Arabidopsis centromeres. Science, 374 (6569). eabi7489. ISSN 0036-8075

Abstract

The centromeres of eukaryotic chromosomes assemble the multiprotein kinetochore complex and thereby position attachment to the spindle microtubules, allowing chromosome segregation during cell division. The key function of the centromere is to load nucleosomes containing the CENTROMERE SPECIFIC HISTONE H3 (CENH3) histone variant [also known as centromere protein A (CENPA)], which directs kinetochore formation. Despite their conserved function during chromosome segregation, centromeres show radically diverse organization between species at the sequence level, ranging from single nucleosomes to megabase-scale satellite repeat arrays, which is termed the centromere paradox. Centromeric satellite repeats are variable in sequence composition and length when compared between species and show a high capacity for evolutionary change, both at the levels of primary sequence and array position along the chromosome. However, the genetic and epigenetic features that contribute to centromere function and evolution are incompletely understood, in part because of the challenges of centromere sequence assembly and functional genomics of highly repetitive sequences. New long-read DNA sequencing technologies can now resolve these complex repeat arrays, revealing insights into centromere architecture and chromatin organization.

Item Type:	Paper
Subjects:	organism description > plant > Arabidopsis bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome > centromere bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome > centromere bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function bioinformatics > genomics and proteomics > genetics & nucleic acid processing > epigenetics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > epigenetics evolution bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation > histone methylation organs, tissues, organelles, cell types and functions > organelles, types and functions > meiosis organs, tissues, organelles, cell types and functions > organelles, types and functions organs, tissues, organelles, cell types and functions organism description > plant
CSHL Authors:	Martienssen, Robert A. Schatz, Michael C.
Communities:	CSHL labs > Martienssen lab CSHL labs > Schatz lab CSHL Cancer Center Program CSHL Cancer Center Program > Gene Regulation and Inheritance Program
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	12 November 2021
Date Deposited:	17 Nov 2021 16:29
Last Modified:	13 Feb 2024 18:52
PMCID:	PMC10164409
URI:	https://repository.cshl.edu/id/eprint/40425

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