Naish, Matthew, Alonge, Michael, Wlodzimierz, Piotr, Tock, Andrew J, Abramson, Bradley W, Schmücker, Anna, Mandáková, Terezie, Jamge, Bhagyshree, Lambing, Christophe, Kuo, Pallas, Yelina, Natasha, Hartwick, Nolan, Colt, Kelly, Smith, Lisa M, Ton, Jurriaan, Kakutani, Tetsuji, Martienssen, Robert A, Schneeberger, Korbinian, Lysak, Martin A, Berger, Frédéric, Bousios, Alexandros, Michael, Todd P, Schatz, Michael C, Henderson, Ian R
(November 2021)
The genetic and epigenetic landscape of the Arabidopsis centromeres.
Science, 374 (6569).
eabi7489.
ISSN 0036-8075
Abstract
The centromeres of eukaryotic chromosomes assemble the multiprotein kinetochore complex and thereby position attachment to the spindle microtubules, allowing chromosome segregation during cell division. The key function of the centromere is to load nucleosomes containing the CENTROMERE SPECIFIC HISTONE H3 (CENH3) histone variant [also known as centromere protein A (CENPA)], which directs kinetochore formation. Despite their conserved function during chromosome segregation, centromeres show radically diverse organization between species at the sequence level, ranging from single nucleosomes to megabase-scale satellite repeat arrays, which is termed the centromere paradox. Centromeric satellite repeats are variable in sequence composition and length when compared between species and show a high capacity for evolutionary change, both at the levels of primary sequence and array position along the chromosome. However, the genetic and epigenetic features that contribute to centromere function and evolution are incompletely understood, in part because of the challenges of centromere sequence assembly and functional genomics of highly repetitive sequences. New long-read DNA sequencing technologies can now resolve these complex repeat arrays, revealing insights into centromere architecture and chromatin organization.
| Item Type: |
Paper
|
| Subjects: |
organism description > plant > Arabidopsis
bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome > centromere
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome > centromere
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > epigenetics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > epigenetics
evolution
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation > histone methylation
organs, tissues, organelles, cell types and functions > organelles, types and functions > meiosis
organs, tissues, organelles, cell types and functions > organelles, types and functions
organs, tissues, organelles, cell types and functions
organism description > plant |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Martienssen lab
CSHL labs > Schatz lab
CSHL Cancer Center Program
CSHL Cancer Center Program > Gene Regulation and Inheritance Program |
| SWORD Depositor: |
CSHL Elements
|
| Depositing User: |
CSHL Elements
|
| Date: |
12 November 2021 |
| Date Deposited: |
17 Nov 2021 16:29 |
| Last Modified: |
13 Feb 2024 18:52 |
| PMCID: |
PMC10164409 |
| URI: |
https://repository.cshl.edu/id/eprint/40425 |
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