Anti-tumor effects of an ID antagonist with no observed acquired resistance.

Wojnarowicz, Paulina M, Escolano, Marta Garcia, Huang, Yun-Han, Desai, Bina, Chin, Yvette, Shah, Riddhi, Xu, Sijia, Yadav, Saurabh, Yaklichkin, Sergey, Ouerfelli, Ouathek, Soni, Rajesh Kumar, Philip, John, Montrose, David C, Healey, John H, Rajasekhar, Vinagolu K, Garland, William A, Ratiu, Jeremy, Zhuang, Yuan, Norton, Larry, Rosen, Neal, Hendrickson, Ronald C, Zhou, Xi Kathy, Iavarone, Antonio, Massague, Joan, Dannenberg, Andrew J, Lasorella, Anna, Benezra, Robert (May 2021) Anti-tumor effects of an ID antagonist with no observed acquired resistance. npj Breast Cancer, 7 (1). p. 58. ISSN 2374-4677

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DOI: 10.1038/s41523-021-00266-0


ID proteins are helix-loop-helix (HLH) transcriptional regulators frequently overexpressed in cancer. ID proteins inhibit basic-HLH transcription factors often blocking differentiation and sustaining proliferation. A small-molecule, AGX51, targets ID proteins for degradation and impairs ocular neovascularization in mouse models. Here we show that AGX51 treatment of cancer cell lines impairs cell growth and viability that results from an increase in reactive oxygen species (ROS) production upon ID degradation. In mouse models, AGX51 treatment suppresses breast cancer colonization in the lung, regresses the growth of paclitaxel-resistant breast tumors when combined with paclitaxel and reduces tumor burden in sporadic colorectal neoplasia. Furthermore, in cells and mice, we fail to observe acquired resistance to AGX51 likely the result of the inability to mutate the binding pocket without loss of ID function and efficient degradation of the ID proteins. Thus, AGX51 is a first-in-class compound that antagonizes ID proteins, shows strong anti-tumor effects and may be further developed for the management of multiple cancers.

Item Type: Paper
Subjects: diseases & disorders > cancer > cancer types > breast cancer
diseases & disorders > cancer > drugs and therapies
CSHL Authors:
Communities: CSHL labs > Wigler lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 24 May 2021
Date Deposited: 01 Jun 2021 19:22
Last Modified: 23 May 2024 13:44
PMCID: PMC8144414

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