Tran, Thu M, Demesa-Arevalo, Edgar, Kitagawa, Munenori, Garcia-Aguilar, Marcelina, Grimanelli, Daniel, Jackson, David (April 2021) An Optimized Whole-Mount Immunofluorescence Method for Shoot Apices. Curr Protoc, 1 (4). e101. ISSN 2691-1299
Abstract
The localization of a protein provides important information about its biological functions. The visualization of proteins by immunofluorescence has become an essential approach in cell biology. Here, we describe an easy-to-follow immunofluorescence protocol to localize proteins in whole-mount tissues of maize (Zea mays) and Arabidopsis. We present the whole-mount immunofluorescence procedure using maize ear primordia and Arabidopsis inflorescence apices as examples, followed by tips and suggestions for each step. In addition, we provide a supporting protocol to describe the use of an ImageJ plug-in to analyze colocalization. This protocol has been optimized to observe proteins in 2-5 mm maize ear primordia or in developing Arabidopsis inflorescence apices; however, it can be used as a reference to perform whole-mount immunofluorescence in other plant tissues and species. © 2021 Wiley Periodicals LLC. Basic Protocol: Whole-mount immunofluorescence for maize and Arabidopsis shoot apices Support Protocol: Measure colocalization by JACoP plugin in ImageJ.
| Item Type: | Paper |
|---|---|
| Subjects: | organism description > plant > Arabidopsis Investigative techniques and equipment organism description > plant > maize Investigative techniques and equipment > microscopy > flourescence microscopy Investigative techniques and equipment > microscopy > immunoflourescence microscopy Investigative techniques and equipment > microscopy |
| CSHL Authors: | |
| Communities: | CSHL labs > Jackson lab |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | April 2021 |
| Date Deposited: | 30 Apr 2021 19:54 |
| Last Modified: | 25 Jan 2024 19:40 |
| URI: | https://repository.cshl.edu/id/eprint/39965 |
Actions (login required)
 |
Administrator's edit/view item |