Yamashiro-Matsumura, S., Kawata, M. (1981) Methionyl Transfer Rna Synthetase Induced Conformational Change of Escherichia-Coli Formyl Methionine Transfer Rna. Journal of Biological Chemistry, 256 (17). pp. 9308-9312.
Abstract
RNase T2, nuclease S1 and snake venom phosphodiesterase were used as structural probes for investigation of the interaction between E. coli tRNAfMet and methionyl-tRNA synthetase, and the cleavage sites were analyzed by a rapid sequencing gel electrophoresis of 5'-32P-labeled tRNA. Both endonucleases cleaved the D-loop of synthetase-bound tRNA much more extensively than that of the free tRNA.
| Item Type: | Paper |
|---|---|
| Subjects: | organism description > bacteria > escherichia coli bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene transfer |
| Communities: | Cold Spring Harbor Laboratory of Quantitative Biology |
| Depositing User: | Elizabeth Pessala |
| Date: | 1981 |
| Date Deposited: | 15 May 2020 21:31 |
| Last Modified: | 15 May 2020 21:31 |
| URI: | https://repository.cshl.edu/id/eprint/39460 |
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