Schreiner, Sarah M., Koo, Peter K., Zhao, Yao, Mochrie, Simon G. J., King, Megan C. (2015) The tethering of chromatin to the nuclear envelope supports nuclear mechanics. Nature Communications, 6. p. 7159.
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Abstract
The nuclear lamina is thought to be the primary mechanical defence of the nucleus. However, the lamina is integrated within a network of lipids, proteins and chromatin; the interdependence of this network poses a challenge to defining the individual mechanical contributions of these components. Here, we isolate the role of chromatin in nuclear mechanics by using a system lacking lamins. Using novel imaging analyses, we observe that untethering chromatin from the inner nuclear membrane results in highly deformable nuclei in vivo, particularly in response to cytoskeletal forces. Using optical tweezers, we find that isolated nuclei lacking inner nuclear membrane tethers are less stiff than wild-type nuclei and exhibit increased chromatin flow, particularly in frequency ranges that recapitulate the kinetics of cytoskeletal dynamics. We suggest that modulating chromatin flow can define both transient and long-lived changes in nuclear shape that are biologically important and may be altered in disease.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromatin remodeling bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cytoskeletal proteins organs, tissues, organelles, cell types and functions > tissues types and functions > lamina |
| CSHL Authors: | |
| Communities: | CSHL labs > Koo Lab |
| Depositing User: | Matthew Dunn |
| Date: | 2015 |
| Date Deposited: | 16 Sep 2019 18:13 |
| Last Modified: | 16 Jul 2021 13:18 |
| PMCID: | PMC4490570 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/38395 |
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