Preparation of plasma membrane from isolated neurons

Henn, F. A., Hansson, H. A., Hamberger, A. (June 1972) Preparation of plasma membrane from isolated neurons. J Cell Biol, 53 (3). pp. 654-61. ISSN 0021-9525 (Print)0021-9525 (Linking)



A bulk fraction enriched with respect to neuronal cell bodies was used as starting material for the isolation of neuronal plasma membrane The cells were gently homogenized in isotonic sucrose and a crude membrane containing fraction sedimented at 3000 g. Subsequently, the membrane fraction was purified on a discontinuous sucrose density gradient between 35% and 25 5% sucrose (w/w). Enzymatic analyses showed a 4-5-fold enrichment in plasma membrane markers, and a 10-15% contamination of mitochondrial and microsomal material. Electron micrographs of the membrane fraction confirmed the enzymatic data Fragmented membranes were found, mainly in vesicular form No ribosomes, but a few mitochondria and some multilamellar membranes were seen

Item Type: Paper
Uncontrolled Keywords:
Subjects: organs, tissues, organelles, cell types and functions > tissues types and functions > cerebral cortex
Investigative techniques and equipment > microscopy > electron microscopy
CSHL Authors:
Communities: Cold Spring Harbor Laboratory of Quantitative Biology
Depositing User: Elizabeth Pessala
Date: June 1972
Date Deposited: 11 Apr 2019 17:01
Last Modified: 11 Apr 2019 17:01
PMCID: PMC2108764

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