Henn, F. A., Hansson, H. A., Hamberger, A. (June 1972) Preparation of plasma membrane from isolated neurons. J Cell Biol, 53 (3). pp. 654-61. ISSN 0021-9525 (Print)0021-9525 (Linking)
Abstract
A bulk fraction enriched with respect to neuronal cell bodies was used as starting material for the isolation of neuronal plasma membrane The cells were gently homogenized in isotonic sucrose and a crude membrane containing fraction sedimented at 3000 g. Subsequently, the membrane fraction was purified on a discontinuous sucrose density gradient between 35% and 25 5% sucrose (w/w). Enzymatic analyses showed a 4-5-fold enrichment in plasma membrane markers, and a 10-15% contamination of mitochondrial and microsomal material. Electron micrographs of the membrane fraction confirmed the enzymatic data Fragmented membranes were found, mainly in vesicular form No ribosomes, but a few mitochondria and some multilamellar membranes were seen
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | |
| Subjects: | organs, tissues, organelles, cell types and functions > tissues types and functions > cerebral cortex Investigative techniques and equipment > microscopy > electron microscopy |
| CSHL Authors: | |
| Communities: | Cold Spring Harbor Laboratory of Quantitative Biology |
| Depositing User: | Elizabeth Pessala |
| Date: | June 1972 |
| Date Deposited: | 11 Apr 2019 17:01 |
| Last Modified: | 11 Apr 2019 17:01 |
| PMCID: | PMC2108764 |
| URI: | https://repository.cshl.edu/id/eprint/37331 |
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