Efrat, S., Teitelman, G., Anwar, M., Ruggiero, D., Hanahan, D.
(September 1988)
Glucagon gene regulatory region directs oncoprotein expression to neurons and pancreatic alpha cells.
Neuron, 1 (7).
pp. 605-13.
ISSN 0896-6273
Abstract
The regulatory region of the rat preproglucagon gene targets expression of the SV40 large T oncoprotein to two cell types in transgenic mice, the pancreatic alpha cells and a set of neurons localized in the hindbrain, both of which normally produce preproglucagon. Additional neurons in the forebrain and midbrain stain for T antigen but do not express the endogenous glucagon gene. Synthesis of T antigen in endocrine alpha cells results in the heritable development of pancreatic glucagonomas. In brains of transgenic mice from three independent lineages, expression of the hybrid gene begins at embryonic day 12 in neuroblasts of the hindbrain, where it continues throughout adult life, most notably in the medulla. Remarkably, oncoprotein expression in both proliferating neuroblasts and mature neurons has no apparent consequences, either phenotypic or tumorigenic. Expression of the hybrid glucagon gene in both neurons and islet cells supports a possible interrelationship between these cell types.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Animals
Antigens, CD5
Antigens, Differentiation/*genetics
Brain/*metabolism
Embryo
*Gene Expression Regulation, Neoplastic
*Genes, Regulator
Glucagon/*genetics
Mice
Mice, Transgenic/*metabolism
Pancreas/*metabolism
Protein Precursors/*genetics
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > pancreatic stellate cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > pancreatic stellate cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > pancreatic stellate cells
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression |
| CSHL Authors: |
|
| Communities: |
CSHL labs |
| Depositing User: |
Gail Sherman
|
| Date: |
September 1988 |
| Date Deposited: |
17 Oct 2017 17:23 |
| Last Modified: |
17 Oct 2017 17:23 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/35142 |
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