Draetta, G., Brizuela, L., Moran, B., Beach, D. (June 1988) Regulation of the vertebrate cell cycle by the cdc2 protein kinase. Cold Spring Harb Symp Quant Biol, 53 Pt . pp. 195-201. ISSN 0091-7451
Abstract
A homolog of the cdc2/CDC28 protein kinase of yeast is found in all vertebrate species that have been investigated. Human cdc2 exists as a complex with a 13-kD protein that is homologous to the suc1 gene product of fission yeast. In both human and fission yeast cells, the protein kinase also exists in a complex with a 62-kD polypeptide that has not been identified genetically but acts as a substrate in vitro. We have studied the properties of the protein kinase in rat and human cells, as well as in Xenopus eggs. We find that in baby rat kidney (BRK) cells, which are quiescent in cell culture, the cdc2 protein is not synthesized. However, synthesis is rapidly induced in response to proliferative activation by infection with adenovirus. In human HeLa cells, the protein kinase is present continuously. It behaves as a cell-cycle oscillator that is inactive in G1 but displays
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals CDC2 Protein Kinase *Cell Cycle Cell Line Humans Phosphoproteins/*metabolism Protein Kinases/*metabolism Saccharomyces cerevisiae/cytology/physiology *Signal Transduction Vertebrates |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell regulation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > Protein kinase C |
| CSHL Authors: | |
| Communities: | CSHL labs > Beach lab |
| Depositing User: | Gail Sherman |
| Date: | June 1988 |
| Date Deposited: | 06 Oct 2017 15:39 |
| Last Modified: | 06 Oct 2017 15:39 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/35137 |
Actions (login required)
 |
Administrator's edit/view item |