Bar-Sagi, D., Gomperts, B. D. (October 1988) Stimulation of exocytotic degranulation by microinjection of the ras oncogene protein into rat mast cells. Oncogene, 3 (4). pp. 463-9. ISSN 0950-9232
Abstract
To investigate the possible role of ras proteins in the secretory process, we have microinjected the proto oncogenic and oncogenic forms of the human H-ras protein into rat peritoneal mast cells. Mast cells are secretory cells which, upon appropriate stimulus, liberate histamine and other mediators of the acute inflammatory reaction by exocytotic degranulation. We report here that microinjection of the ras oncogene protein into mast cells induces exocytotic degranulation. In contrast, microinjection of similar amounts of the proto-oncogenic protein has little apparent effect on mast cells. Degranulation induced by injection of the ras oncogene protein occurs in the absence of an external stimulus and requires the presence of external calcium. The ultrastructural features of exocytotic degranulation in mast cells injected with the ras oncogene protein are similar to those seen when mast cells are activated by soluble ligands. Our results suggest that ras proteins may be involved, possibly as regulatory elements, in cellular functions that control exocytosis.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Cytoplasmic Granules/ultrastructure *Exocytosis *Genes, ras Humans Kinetics Mast Cells/*physiology/ultrastructure Microinjections Microscopy, Electron Oncogene Protein p21(ras)/administration & dosage/*metabolism Rats Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Time Factors |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > G protein > Ras organism description > animal > mammal > rodent > rat organism description > animal > mammal > rodent > rat |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Gail Sherman |
| Date: | October 1988 |
| Date Deposited: | 10 Nov 2017 16:44 |
| Last Modified: | 10 Nov 2017 16:44 |
| URI: | https://repository.cshl.edu/id/eprint/35117 |
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