Phosphorylation of large tumour antigen by cdc2 stimulates SV40 DNA replication

McVey, D., Brizuela, L., Mohr, I., Marshak, D. R., Gluzman, Y., Beach, D. (October 1989) Phosphorylation of large tumour antigen by cdc2 stimulates SV40 DNA replication. Nature, 341 (6242). pp. 503-7. ISSN 1476-4687

Abstract

Simian virus 40 large tumour antigen (T) is a replication origin binding protein required for viral DNA synthesis. Unphosphorylated T antigen is deficient in promoting DNA replication in vitro but can be activated by phosphorylation at residue threonine 124 by the cdc2 protein kinase. This observation demonstrates that T is regulated by phosphorylation and provides a model for cdc2 function in the control of DNA replication.

Item Type: Paper
Uncontrolled Keywords: *Antigens, Polyomavirus Transforming/genetics/isolation & purification CDC2 Protein Kinase *DNA Replication DNA-Binding Proteins/metabolism Escherichia coli/genetics Hela Cells/enzymology Humans Kinetics Phosphoproteins/*metabolism Phosphorylation Protein Kinases/*metabolism Research Support, U.S. Gov't, P.H.S. Simian virus 40/*genetics/immunology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cdc2
organism description > virus
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Gail Sherman
Date: 12 October 1989
Date Deposited: 27 Jun 2017 18:26
Last Modified: 27 Jun 2017 18:26
Related URLs:
URI: https://repository.cshl.edu/id/eprint/34879

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