Cohen, D. R., Ferreira, P. C. P., Gentz, R., Franza, B. R., Curran, T. (February 1989) The Product of a Fos-Related Gene, Fra-1, Binds Cooperatively to the Ap-1 Site with Jun - Transcription Factor Ap-1 Is Comprised of Multiple Protein Complexes. Genes & Development, 3 (2). pp. 173-184. ISSN 0890-9369
Abstract
fra-1 encodes a serum-inducible protein (Fra-1) that is antigenically related to Fos. We have characterized Fra-1 expression in serum-stimulated cells using antibodies raised against several regions of this protein. Fra-1, expressed transiently in COS cells or in serum-stimulated rat fibroblasts, undergoes extensive post-translational modification, primarily by phosphorylation of serine residues. It is present in both the nucleus and the cytoplasm and participates in a protein complex with Jun. Using proteins synthesized in reticulocyte lysates, we have shown that Fra-1, like Fos, binds to the AP-1 recognition element cooperatively with Jun. A truncated Fra-1 protein that contains the leucine zipper region but not an adjacent basic amino acid domain, complexes with Jun in vitro but fails to bind AP-1 oligonucleotides. These results demonstrate that Fra-1 contributes to the DNA-binding activity ascribed to transcription factor AP-1.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Gail Sherman |
| Date: | February 1989 |
| Date Deposited: | 31 Jul 2017 17:52 |
| Last Modified: | 31 Jul 2017 17:52 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/34838 |
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