Stamm, S., Casper, D., Lees-Miller, J. P., Helfman, D. M. (November 1993) Brain-specific tropomyosins TMBr-1 and TMBr-3 have distinct patterns of expression during development and in adult brain. Proc Natl Acad Sci U S A, 90 (21). pp. 9857-61. ISSN 0027-8424 (Print)
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Abstract
In this study we report on the developmental and regional expression of two brain-specific isoforms of tropomyosin, TMBr-1 and TMBr-3, that are generated from the rat alpha-tropomyosin gene via the use of alternative promoters and alternative RNA splicing. Western blot analysis using an exon-specific peptide polyclonal antibody revealed that the two isoforms are differentially expressed in development with TMBr-3 appearing in the embryonic brain at 16 days of gestation, followed by the expression of TMBr-1 at 20 days after birth. TMBr-3 was detected in all brain regions examined, whereas TMBr-1 was detected predominantly in brain areas that derived from the prosencephalon. Immunocytochemical studies on mixed primary cultures made from rat embryonic midbrain indicate that expression of the brain-specific epitope is restricted to neurons. The developmental pattern and neuronal localization of these forms of tropomyosin suggest that these isoforms have a specialized role in the development and plasticity of the nervous system.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Aging/ metabolism Amino Acid Sequence Animals Antibodies Base Sequence Brain/embryology/growth & development/ metabolism DNA Primers Embryonic and Fetal Development Epitopes/analysis Exons Gene Expression Regulation/ physiology Gestational Age Immunoblotting Kidney/metabolism Liver/metabolism Molecular Sequence Data Muscles/metabolism Neurons/ metabolism Organ Specificity Peptides/chemical synthesis/immunology Polymerase Chain Reaction Rats Rats, Sprague-Dawley Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Tropomyosin/analysis/ biosynthesis |
| Subjects: | organs, tissues, organelles, cell types and functions > organs types and functions > brain bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > exons bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > tropomyosin |
| CSHL Authors: | |
| Communities: | CSHL labs > Helfman lab |
| Depositing User: | Matt Covey |
| Date: | 1 November 1993 |
| Date Deposited: | 08 Apr 2016 20:08 |
| Last Modified: | 09 Nov 2017 19:43 |
| PMCID: | PMC47671 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32592 |
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