Walworth, N., Davey, S., Beach, D. (May 1993) Fission yeast chk1 protein kinase links the rad checkpoint pathway to cdc2. Nature, 363 (6427). pp. 368-71. ISSN 0028-0836 (Print)
Abstract
The dependence of cell-cycle progression on the integrity of the genome has been described as checkpoint control. A number of mutants of the fission yeast Schizosaccharomyces pombe, selected for their sensitivity to DNA damage caused by radiation (rad mutants) or to the DNA synthesis inhibitor hydroxyurea (hus mutants) have been classified as checkpoint mutants because they fail to arrest the cell cycle in response to DNA damage or incompletely replicated DNA. Coupling of the checkpoint pathways that monitor DNA repair and replication to control of the cell cycle is essential. In a search for components that interact with the cell-cycle regulatory kinase p34cdc2, we have identified a novel fission yeast protein kinase homologue which is involved in cell-cycle arrest when DNA damage has occurred or when unligated DNA is present. We have called the gene encoding this protein chk1 for checkpoint kinase. Multiple copies of chk1 partially rescue the ultraviolet sensitivity of rad1-1, a mutant deficient in checkpoint control. Identification of a gene involved in checkpoint control as a rescue of a cdc2 mutant links the rad1-dependent DNA-damage-sensing pathway and p34cdc2 activity.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence CDC2 Protein Kinase/ metabolism Cell Cycle/ genetics DNA Damage DNA, Fungal/drug effects/genetics DNA-Binding Proteins Endonucleases Fungal Proteins/genetics/ metabolism Genes, Fungal Hydroxyurea/pharmacology Molecular Sequence Data Mutation Protein Kinases/genetics/ metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Restriction Mapping Schizosaccharomyces/cytology/ enzymology/genetics Schizosaccharomyces pombe Proteins |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cdc2 organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Beach lab |
| Depositing User: | Matt Covey |
| Date: | 27 May 1993 |
| Date Deposited: | 12 Apr 2016 19:22 |
| Last Modified: | 12 Apr 2016 19:22 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32577 |
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