Samejima, I., Matsumoto, T., Nakaseko, Y., Beach, D., Yanagida, M. (May 1993) Identification of 7 New Cut Genes Involved in Schizosaccharomyces-Pombe Mitosis. Journal of Cell Science, 105. pp. 135-143. ISSN 0021-95331477-9137
Abstract
Fission yeast cut mutants cause cytokinesis in the absence of normal nuclear division. These mutants show abnormal uncoupled mitosis and are known to be the result of mutations in the genes encoding DNA topoisomerase II, proteins related to spindle pole duplication, and a kinesin-related mitotic motor. We have screened 717 temperature-sensitive (ts) mutants by individually observing their cytological phenotypes at the restrictive temperature, and have newly isolated 25 cut mutants. Genetic analyses indicate that 14 of them fall into five previously identified loci, namely, top2, cut1, cut5, cut7 and cut9, whereas nine have been mapped onto seven new loci, designated cut13 to cut19. The cytological phenotypes of the newly identified cut mutants can be classified into three groups. One group consists of mutants in which a portion of the nuclear chromatin is stretched by the elongated spindle but the entire nucleus is not separated, reminiscent of, but not identical to, the phenotypes of top2 and cut1; mutants cut14-208, cut15-85, cut16-267 and cut17-275 display such a phenotype. Another group exhibits non-disjunctioned and condensed chromosomes in the presence of the spindle, cut13-131 belongs to this group. The cut19-708 mutant has also been found to have condensed chromosomes. The remaining group has a mixed phenotype of the above two groups; namely, stretched chromatin and condensed chromosomes; cut18-447 exhibits such a phenotype. The isolation and characterization of the mutated genes will be the subjects of future investigations.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | MITOSIS CUT MUTANT FISSION YEAST DNA TOPOISOMERASE-II MOTOR PROTEIN SPINDLE MOVEMENT DNA TOPOISOMERASE-II FISSION YEAST SACCHAROMYCES-CEREVISIAE MUTANTS DUPLICATION CHROMOSOMES SEPARATION DIVISION ENCODES BINDING |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Beach lab |
| Depositing User: | Matt Covey |
| Date: | May 1993 |
| Date Deposited: | 18 Apr 2016 20:51 |
| Last Modified: | 18 Apr 2016 20:51 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32532 |
Actions (login required)
 |
Administrator's edit/view item |