Lundgren, K., Walworth, N., Booher, R., Dembski, M., Kirschner, M., Beach, D. (March 1991) mik1 and wee1 cooperate in the inhibitory tyrosine phosphorylation of cdc2. Cell, 64 (6). pp. 1111-22. ISSN 0092-8674 (Print)0092-8674 (Linking)
Abstract
wee1 acts antagonistically to cdc25 in the tyrosine dephosphorylation and activation of cdc2, yet biochemical evidence suggests that wee1 is not required for tyrosine phosphorylation and its role is obscure. We show here that a related 66 kd kinase, called mik1, acts redundantly with wee1 in the negative regulation of cdc2 in S. pombe. A null allele of mik1 has no discernible phenotype, but a mik1 wee1 double mutant is hypermitotically lethal: all normal M phase checkpoints are bypassed, including the requirement for initiation of cell cycle "start," completion of S phase, and function of the cdc25+ mitotic activator. In the absence of mik1 and wee1 activity, cdc2 rapidly loses phosphate on tyrosine, both in strains undergoing mitotic lethality and in those that are viable owing to a compensating mutation within cdc2. The data suggest that mik1 and wee1 act cooperatively on cdc2, either directly as the inhibitory tyrosine kinase or as essential activators of that kinase.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Base Sequence CDC2 Protein Kinase/genetics/*metabolism Cloning, Molecular Genes, Fungal/physiology Mitosis/*genetics Molecular Sequence Data Mutation Phosphorylation Phosphotyrosine Schizosaccharomyces/cytology/genetics Sequence Homology, Nucleic Acid Tyrosine/analogs & derivatives/metabolism |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cdc2 bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > tyrosine kinase |
| CSHL Authors: | |
| Communities: | CSHL labs > Beach lab |
| Depositing User: | Matt Covey |
| Date: | 22 March 1991 |
| Date Deposited: | 21 Dec 2015 21:43 |
| Last Modified: | 21 Dec 2015 21:43 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32109 |
Actions (login required)
 |
Administrator's edit/view item |