Wang, P. C., Vancura, A., Mitcheson, T. G., Kuret, J. (March 1992) Two genes in Saccharomyces cerevisiae encode a membrane-bound form of casein kinase-1. Mol Biol Cell, 3 (3). pp. 275-86. ISSN 1059-1524 (Print)1059-1524 (Linking)
Abstract
Two cDNAs encoding casein kinase-1 have been isolated from a yeast cDNA library and termed CKI1 and CKI2. Each clone encodes a protein of approximately 62,000 Da containing a highly conserved protein kinase domain surrounded by variable amino- and carboxy-terminal domains. The proteins also contain two conserved carboxy-terminal cysteine residues that comprise a consensus sequence for prenylation. Consistent with this posttranslational modification, cell fractionation experiments demonstrate that intact CKI1 is found exclusively in yeast cell membranes. Gene disruption experiments reveal that, although neither of the two CKI genes is essential by itself, at least one CKI gene is required for yeast cell viability. Spores deficient in both CKI1 and CKI2 fail to grow and, therefore, either fail to germinate or arrest as small cells before bud emergence. These results suggest that casein kinase-1, which is distributed widely in nature, plays a pivotal role in eukaryotic cell regulation.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Base Sequence Casein Kinases Cell Membrane/enzymology Chromosome Mapping Cloning, Molecular DNA, Fungal/chemistry/genetics/isolation & purification Genes, Fungal/*genetics Molecular Sequence Data Phenotype Protein Kinases/chemistry/*genetics/isolation & purification Saccharomyces cerevisiae/enzymology/*genetics |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | March 1992 |
| Date Deposited: | 29 Sep 2015 15:11 |
| Last Modified: | 29 Sep 2015 15:11 |
| PMCID: | PMC275529 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31819 |
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