miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1

Zhang, L., Flygare, J., Wong, P., Lim, B., Lodish, H. F. (January 2011) miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1. Genes Dev, 25 (2). pp. 119-24. ISSN 1549-5477 (Electronic)0890-9369 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/21196494

DOI: 10.1101/gad.1998711

Abstract

Using RNA-seq technology, we found that the majority of microRNAs (miRNAs) present in CFU-E erythroid progenitors are down-regulated during terminal erythroid differentiation. Of the developmentally down-regulated miRNAs, ectopic overexpression of miR-191 blocks erythroid enucleation but has minor effects on proliferation and differentiation. We identified two erythroid-enriched and developmentally up-regulated genes, Riok3 and Mxi1, as direct targets of miR-191. Knockdown of either Riok3 or Mxi1 blocks enucleation, and either physiological overexpression of miR-191 or knockdown of Riok3 or Mxi1 blocks chromatin condensation. Thus, down-regulation of miR-191 is essential for erythroid chromatin condensation and enucleation by allowing up-regulation of Riok3 and Mxi1.

Item Type:	Paper
Uncontrolled Keywords:	Animals Basic Helix-Loop-Helix Transcription Factors/genetics/ metabolism Cell Differentiation Cell Line Cell Nucleus/metabolism Cells, Cultured Down-Regulation Erythroblasts/ cytology/metabolism Gene Knockdown Techniques Mice MicroRNAs/ metabolism Protein-Serine-Threonine Kinases/ metabolism Tumor Suppressor Proteins/genetics/ metabolism Up-Regulation
Subjects:	organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell differentiation organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell proliferation organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell differentiation > erythropoiesis bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA Investigative techniques and equipment > assays > RNA-seq
CSHL Authors:	Zhang, Lingbo
Communities:	CSHL labs > Zhang L. lab
Depositing User:	Matt Covey
Date:	15 January 2011
Date Deposited:	13 Jul 2015 20:43
Last Modified:	03 Nov 2017 20:08
PMCID:	PMC3022257
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/31620

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