Xu, C., Liberatore, K. L., MacAlister, C. A., Huang, Z., Chu, Y. H., Jiang, K., Brooks, C., Ogawa-Ohnishi, M., Xiong, G., Pauly, M., Van Eck, J., Matsubayashi, Y., van der Knaap, E., Lippman, Z. B. (July 2015) A cascade of arabinosyltransferases controls shoot meristem size in tomato. Nat Genet, 47 (7). pp. 784-792. ISSN 1546-1718 (Electronic)1061-4036 (Linking)
Abstract
Shoot meristems of plants are composed of stem cells that are continuously replenished through a classical feedback circuit involving the homeobox WUSCHEL (WUS) gene and the CLAVATA (CLV) gene signaling pathway. In CLV signaling, the CLV1 receptor complex is bound by CLV3, a secreted peptide modified with sugars. However, the pathway responsible for modifying CLV3 and its relevance for CLV signaling are unknown. Here we show that tomato inflorescence branching mutants with extra flower and fruit organs due to enlarged meristems are defective in arabinosyltransferase genes. The most extreme mutant is disrupted in a hydroxyproline O-arabinosyltransferase and can be rescued with arabinosylated CLV3. Weaker mutants are defective in arabinosyltransferases that extend arabinose chains, indicating that CLV3 must be fully arabinosylated to maintain meristem size. Finally, we show that a mutation in CLV3 increased fruit size during domestication. Our findings uncover a new layer of complexity in the control of plant stem cell proliferation.
| Item Type: | Paper |
|---|---|
| Subjects: | organs, tissues, organelles, cell types and functions > tissues types and functions > meristem organism description > plant organism description > plant > tomato |
| CSHL Authors: | |
| Communities: | CSHL labs > Lippman lab School of Biological Sciences > Publications |
| Depositing User: | Matt Covey |
| Date: | July 2015 |
| Date Deposited: | 27 May 2015 20:44 |
| Last Modified: | 15 Jul 2015 20:46 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31534 |
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