Isolation and characterization of a maize gene encoding chalcone flavonone isomerase

Grotewold, E., Peterson, T. (January 1994) Isolation and characterization of a maize gene encoding chalcone flavonone isomerase. Mol Gen Genet, 242 (1). pp. 1-8. ISSN 0026-8925 (Print)

DOI: 10.1007/BF00277341


We report here the first cloning of a chalcone flavonone isomerase gene (CHI) from maize. Northern blot experiments indicate that the maize CHI gene (ZmCHI1) is regulated in the pericarp by the P gene, a myb homologue. The ZmCHI1 gene encodes a 24.3 kDa product 55% and 58% identical to CHI-A and CHI-B from Petunia, respectively. This maize CHI1 gene has four exons and an intron-exon structure identical to the CHI-B gene of Petunia hybrida. RFLP mapping data indicate that some inbred lines contain two additional CHI-homologous sequences, suggesting an organization more complex than that found in Petunia or bean. The possibility that the additional CHI-homologous sequences are responsible for the lack of CHI mutants in maize will be discussed.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Chalcone/metabolism Cloning, Molecular Comparative Study Conserved Sequence DNA Primers DNA-Binding Proteins/genetics Fabaceae/genetics Flavonoids/ metabolism Gene Expression Regulation, Enzymologic Genes, Plant Genes, Regulator Intramolecular Lyases Isomerases/ genetics Molecular Sequence Data Mutation Pigmentation/genetics Plant Proteins/ genetics Plants/genetics Plants, Medicinal Restriction Mapping Sequence Alignment Sequence Analysis, DNA Sequence Homology, Nucleic Acid Zea mays/enzymology/ genetics
Subjects: organism description > plant > maize
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
CSHL Authors:
Communities: CSHL labs > Grotewold lab
Depositing User: Matt Covey
Date: January 1994
Date Deposited: 04 May 2015 14:39
Last Modified: 04 May 2015 14:39
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